big dye sequence accuracy

From: LSOUTO (LSOUTO@bio.ua.pt)
Date: Thu Feb 17 2000 - 12:56:36 EST


Thank you for the help in previous question.
This list is ideal for talking with people with lot of experience.
I would appreciate your comments on these questions:

1-When running my sequence-samples (Big Dye Term) on ABI 377, i notice
problems whenever there is a repeat of the same base. That is, if the
consecutive bases are different, the peaks are well defined, but if the
bases are the same there's a lack of definition
2- If I analyse with ABI200 the software miss many of the (badly separated)
peaks. However using ABI semi-adaptative, the result is completely
different. What is the correct one?

3- is there any significant difference between the 2400 and 1200 (bph, i.e.,

running 3 or 6 hours?)

  



This archive was generated by hypermail 2b29 : Thu Mar 16 2000 - 10:06:51 EST