I found that lowering the heated capillary by as much as 100 C from the
suggested temperature helped significantly, especially when running at high
flow rates. I've run proteins as large as 80K with very good sensitivity.
John
> ----------
> From: Rong-Rong Zhu[SMTP:zhur@basf-corp.com]
> Sent: Thursday, February 17, 2000 7:36 AM
> To: Recipients of ABRF List
> Cc: Kathleen L Grant
>
> Has anyone run intact protein with molecular weight larger than 40 k on
> LCQ-Deca? We just had our LCQ-deca installed and we have some s/n problem
> especially when we are working with intact proteins. The TIC value is
> much
> higher from Deca than from LCQ- classic but the s/n ratio is much poorer.
> The
> averaged spectra is so poor that we can not do any deconvolution for
> protein
> larger than 40 k. I am wondering if this is just our LCQ-deca problem or
> if it
> is a problem with all LCQ-deca.
>
>
> Rong-Rong Zhu
> BASF Bioresearch Corp.
> 508-849-2770
> zhur@basf.com
>
>
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