I have a question related to protein MW determination by LCQ for direct
sample infusion.
For a protein of 80 Kda, what is the concentration required to determine
the molecular weight (assume protein in a solution without salt or
glycerol).
Thank you.
yanni
> I found that lowering the heated capillary by as much as 100 C from the
> suggested temperature helped significantly, especially when running at high
> flow rates. I've run proteins as large as 80K with very good sensitivity.
>
> John
>
> > ----------
> > From: Rong-Rong Zhu[SMTP:zhur@basf-corp.com]
> > Sent: Thursday, February 17, 2000 7:36 AM
> > To: Recipients of ABRF List
> > Cc: Kathleen L Grant
> >
> > Has anyone run intact protein with molecular weight larger than 40 k on
> > LCQ-Deca? We just had our LCQ-deca installed and we have some s/n problem
> > especially when we are working with intact proteins. The TIC value is
> > much
> > higher from Deca than from LCQ- classic but the s/n ratio is much poorer.
> > The
> > averaged spectra is so poor that we can not do any deconvolution for
> > protein
> > larger than 40 k. I am wondering if this is just our LCQ-deca problem or
> > if it
> > is a problem with all LCQ-deca.
> >
> >
> > Rong-Rong Zhu
> > BASF Bioresearch Corp.
> > 508-849-2770
> > zhur@basf.com
> >
> >
>
>
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