Re: IEF

From: Norman Watts (WATTS@calvin.niams.nih.gov)
Date: Tue Feb 22 2000 - 08:45:38 EST

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WRT the IEF/transfer problem

I did IEF like that for my thesis. See Watts and Coombs
1989. J. Vir. 63:2427; Watts and Coombs 1990. J. Vir.
64:143; Watts, Hainfeld and Coombs 1990. J. Mol. Biol.
216:315; Watts and Singh, 1995. Electrophoresis 16:22.
Maybe there is some help there.

Also, do you have (a) to high ionic strength? (b) bad
current? i.e. check wires, try another power supply.

I don't think a gradient gel will affect transfer, just
resolution and separation range.

good luck

------------------------------------------------------------

Norman Watts, PhD.
Laboratory of Structural Biology
NIAMS, National Institutes of Health
Bethesda, MD 20892-2717
U.S.A.
E-mail: watts@calvin.niams.nih.gov
Phone: 301-402-3418
Fax: 301-480-7629

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