Hello,
I was wondering if anyone out there could offer some advice on cyclic peptides. We are currently working on a project that involves making a cyclic peptide in which either the C or N terminus would be biotinylated. My concerns are:
I believe I will need the free amine group to cyclize the peptide and therefore will not have a free amine group to biotinlate.
In addition, both the C and N terminus have a Cysteine. We were thinking that we might be able to cyclize the peptide by forming a disulfide bond between the two ends. That way, we might be able to use the free amine to biotinylate. If this is a possibility, how do we selectively have the single peptide form a disulfide bond between its two ends rather than having a chain formation.
I thank you for any advice,
Jen
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