I ave recently synthesized a peptide with the following aa:
2G N and C terminal
2S
2R
1F
1T
1L
1E
MW 1109.2. The HPLC looks good. Synthesis was in the Perseptive Pioneer. Coupling and deprotection look fine. Mass spec gives a good peak with a mw of 1123.7. I sequenced the peptide on a procise (glass membrane). The sequence died after 8aa which doesnt surprise me. The calls were 100% for the first 8. What do I have? Why is the mw off by 15?
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