I ave recently synthesized a peptide with the following aa:
2G N and C terminal
2S
2R
1F
1T
1L
1E
MW 1109.2. The HPLC looks good. Synthesis was in the Perseptive Pioneer.
Coupling and deprotection look fine. Mass spec gives a good peak with a mw of
1123.7. I sequenced the peptide on a procise (glass membrane). The sequence
died after 8aa which doesnt surprise me. The calls were 100% for the first 8.
What do I have? Why is the mw off by 15?
***********************************************************
Terry,
PE Tech support has said that if the resin is not dried properly, methyl esters
could form with the final methanol wash, during cleavage. We now use Iso
propanol.
Radhika
City of Hope, Duarte.
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