ABRFer's,
I need to get a sequence of a protein that is known to have an N-acetyl
methionine as the N-terminal residue. We usually get internal sequence
information on blocked proteins but I am willing to try a deblocking
protocol, especially since I know what the blocking group is. Does anyone
know of a procedure to remove the N-acetyl group from methionine? Is there
some literature that I can get about deblocking in general?
Thanks,
Bill
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Bill Enslow
Protein Sequencing Technician
Cornell BioResource Center
Rm 143 Biotechnology Building
phone: 607-254-4848
fax: 607-254-4847
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