Dear Terry
You may have a methyl ester. Did you do the methanol wash on your Pioneer
? I suggest you resynthesise it, and leave out the methanol washing
permanently !!
Graham Bloomberg
University of Bristol
UK
On Thu, 24 Feb 2000, Terry Stoming wrote:
> I ave recently synthesized a peptide with the following aa:
> 2G N and C terminal
> 2S
> 2R
> 1F
> 1T
> 1L
> 1E
> MW 1109.2. The HPLC looks good. Synthesis was in the Perseptive Pioneer. Coupling and deprotection look fine. Mass spec gives a good peak with a mw of 1123.7. I sequenced the peptide on a procise (glass membrane). The sequence died after 8aa which doesnt surprise me. The calls were 100% for the first 8. What do I have? Why is the mw off by 15?
>
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