"'Terry Stoming'"@
<TSTOMING@MAIL.MCG.EDU>
Subject: RE: Peptide??
Date: Thu, 24 Feb 2000 10:37:09 -0600
MIME-Version: 1.0
X-Mailer: Internet Mail Service (5.5.2448.0)
Content-Type: text/plain
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sender: Association of Biomolecular Resource Facilities <abrf-request@aecom.yu.edu>
Precedence: bulk
Errors-To: abrf-request@aecom.yu.edu
Could you have formed Me-ester (+14 diff. in MW)? We have seen this if we
used methanol flush in pioneer, so we have eliminated its use. Sequencing
gurus out there may be able to tell you why sequencing stalled out.
Randy Wilhelm
Sr. Research Chemist
Mallinckrodt, Inc
Discovery Research Peptide Team
Phone (314)654-3965
Fax (314)654-8911
rrwilhe@mkg.com
> ----------
> From: Terry Stoming[SMTP:TSTOMING@MAIL.MCG.EDU]
> Sent: Thursday, February 24, 2000 3:37 AM
> To: Recipients of ABRF List
> Subject: Peptide??
>
> I ave recently synthesized a peptide with the following aa:
> 2G N and C terminal
> 2S
> 2R
> 1F
> 1T
> 1L
> 1E
> MW 1109.2. The HPLC looks good. Synthesis was in the Perseptive Pioneer.
> Coupling and deprotection look fine. Mass spec gives a good peak with a
> mw of 1123.7. I sequenced the peptide on a procise (glass membrane). The
> sequence died after 8aa which doesnt surprise me. The calls were 100% for
> the first 8. What do I have? Why is the mw off by 15?
>
This archive was generated by hypermail 2b29 : Thu Mar 16 2000 - 10:06:51 EST