Dear Owen:
Here a list of manufacturers who produce dedicated systems for Microbore
HPLC. To understand that we talk about the same thing here a short
definition of Microbore
1.0 mm I.D. columns with a core flow rate of 40 ul/min for RP column even
so many people run them at 50 ul/min
800 um I.D. columns with a core flow rate of 25 ul/min
Please understand that there is not only the issue of solvent delivery but
also of detection. For utmost efficiency in absorebence detection (UV) the
flow cell should be small enough to prevent extra-column band broadening.
This means cell volumes of 500 ul or less.
Further think also of what happens when you generate a gradient: assuming
you start with 5% B at 40ul/min total flow, your system must be able to
reproducible deliver and mix 2 ul/min.
What I find interesting in your listing of manufacturers is that you do not
mention a single one who builds dedicated micro systems. But then this is
why you are asking the question.
Now here my list which I present without value. The order is not a
recommendation but totally random. First I name the manufacturer then the
model:
Michrom: Galaxy 2000
MicroTech: UltraChem III
Isco: syringe pump system (I'm not sure if it is still available)
ABI: 140 series
Waters: Cap LC -- micro is at the upper end of their range
Eldex: Micro syringe pump
and last but not least our own product LC Packings: UltiMate
Hope that helps -- if you need addresses check the LC/GC Buyer's Guide.
Best regards: Jean-Pierre
----------------------------------------------------
Jean-Pierre Salzmann
Project Manager
LC PACKINGS (USA) INC
80 Carolina Street
San Francisco, CA 94103
Phone 415-552-1855
Fax 415-552-1859
e-mail jps@lcpackingsusa.com
www.lcpackings.com
----------------------------------------------------
----------
>From: Owen Nadeau <nadeauo@umkc.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Microbore HPLC Systems
>Date: Tue, Feb 29, 2000, 1:37 PM
>
>We are currently in the market for a microbore HPLC system and are
>looking at systems by Shimatzu, Beckman and HP among others. We would
>appreciate any input from current users of these systems or users of
>other systems. Thanks in advance.
>
>Owen W. Nadeau
>Dept of Biochemistry and Molecular Biology
>Univ. of MO
>Kansas City, MO
>email: nadeauo@umkc.edu
>
>
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