Acetic anhydride in pyridine is quite a mild method; however if you do
not believe it, you can activate acetic acid like any amino acid (e.g.
with a carbodiimide).
Regards
Vladimir
On 02/03/2000, Michael Jobling wrote:
> Hi Everybody.
> I've got a peptide that I need to acetylate the n-terminus. It has been
> cleaved from the resin and is HPLC clean. It's sequence is:
> KTNMKHMAGAAAAGAVVGGLG. The c-terminus is already amidated. I'm concerned
> that any procedure will affect the side chain of either lysine or the
> histidine or maybe oxidise the methionines. Does anybody have any
> suggestions how I can selectively add this acetyl group?
> Thanks in advance
Vladimir Titov
Bokiron Ltd., Moscow, Russia
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