Phred is an alternative basecaller
(http://www.genome.washington.edu/UWGC/analysistools/phred.htm and
http://www.phrap.com/). Rn phred against your sample files once they
have been extracted. Only drawback is no ABI files, only SCF (which
would be great if there was a high quality free viewer for macs -
anyone know of one?). All reports I have heard is that it works fine
with 3700 data. I believe it has been re-trained with 3700 and
MegaBace data.
-James
>Hi Everyone,
>
>Does anyone know of any alternative basecallers for the ABI 3700's?
>
>Perkin-Elmer claims that they only have the one default basecaller.
>
>Are there any commercially available, or ones that people have created
>themselves that I can either hack or buy??
>
>I was also curious if the MegaBace basecallers would work on the 3700's.
>
>Does anyone know?
>
>Chris Jacobs
>
>DNA Sequencing Lab
>Parke-Davis Pharmaceuticals
>Office Phone: (734) 622-5724
>Lab Phone: (734) 622-1815
>
>E-mail: chris.jacobs@wl.com
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James VanEe Phone: (607) 254-4862
BioResource Center
Computing Facilty
170/171 Biotech Bldg Fax: (607) 254-4847
Cornell University
Ithaca, NY 14853 www: http://brcweb.bio.cornell.edu
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