Michael,
I guess I'm not telling you anything you don't know when I say that you
really should have acetylated the N-terminal while still on the resin -
very simple and straightforward.
However, I did do some derivatisation of alpha-N of lysine once upon a long
time ago by complexing the epsilon-N as (I think) a copper salt. You might
try J. Chem. Soc. (Perkin I) 449-454 (1982) as a starting point, but I
think I found the method in a book series called The Amino Acids.
This may or may not be applicable to your peptide, but it might be quicker
to resynthesise and end-cap with acetic anhydride.!
Cheers,
Roger
At 12:09 PM 3/2/00 +1100, you wrote:
>Hi Everybody.
>
>I've got a peptide that I need to acetylate the n-terminus. It has been
>cleaved from the resin and is HPLC clean. It's sequence is:
>KTNMKHMAGAAAAGAVVGGLG. The c-terminus is already amidated. I'm concerned
>that any procedure will affect the side chain of either lysine or the
>histidine or maybe oxidise the methionines. Does anybody have any
>suggestions how I can selectively add this acetyl group?
>
>Thanks in advance
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au
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