Sorry I didn't notice it has been cleaved from the resin. Now you
can't help it.
Vladimir
On 03/03/2000, neil brew wrote:
> Vladimir,
> Pardon my ignorance, but how is this acetylation method specific to the N
> terminus of the peptide? Surely the lysine side chains will also be
> acetylated?
> -----Original Message-----
> From: Vladimir Titov [mailto:vmtitov@aha.ru]
> Sent: Thursday, March 02, 2000 7:14 PM
> To: Recipients of ABRF List
> Cc: abrf@aecom.yu.edu
> Subject: Re: PepSyn; acetylation
> Acetic anhydride in pyridine is quite a mild method; however if you do
> not believe it, you can activate acetic acid like any amino acid (e.g.
> with a carbodiimide).
> Regards
> Vladimir
> On 02/03/2000, Michael Jobling wrote:
>> Hi Everybody.
>> I've got a peptide that I need to acetylate the n-terminus. It has been
>> cleaved from the resin and is HPLC clean. It's sequence is:
>> KTNMKHMAGAAAAGAVVGGLG. The c-terminus is already amidated. I'm concerned
>> that any procedure will affect the side chain of either lysine or the
>> histidine or maybe oxidise the methionines. Does anybody have any
>> suggestions how I can selectively add this acetyl group?
>> Thanks in advance
> Vladimir Titov
> Bokiron Ltd., Moscow, Russia
Vladimir Titov
vmtitov@aha.ru
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