One caveat:
Depending on the structure of the peptide, it may stick to the .2 um
filter. You should check this as it may impact your experiment.
Joseph
Vladimir Titov wrote:
> I think filtration is the method of choice; you may also add some
> preservative like benzalkonium chloride to your sterile solution.
> Benzalkonium chloride is a standard additive to nasal peptide drugs
> used in medicine; I hope it wouldn't spoil a solution to be injected
> into the animal either.
>
> Vladimir
>
> On 02/03/2000, Alain LAURENT wrote:
>
> > Hello
>
> > I would be interested to know a method to sterilize a peptide for
> > in-vivo experiments. I was told about irradiation or filtration or
> > easier ?
>
> > What do you recommend ?
>
> > Thanks for your help
>
> > ALin
>
> > **************************************
> > Alain LAURENT Ph. D
> > DNA&Peptide Chemistry
> > ESGS Groupe CYBERGENE
> > 11, rue Claude Bernard
> > 35 400 Saint MAL0
> > FRANCE
> > Tel: +33 (0)2 99 21 90 40
> > Fax: +33 (0)2 99 21 90 41
> > e-mail: a.laurent@eurosequence.com
> > http://www.eurosequence.com
> > **************************************
>
> Vladimir Titov
>
> Bokiron Ltd., Moscow, Russia
>
> vmtitov@aha.ru
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Joseph A. Fuselier Peptide Research, SL12 Tulane University School of Medicine 1430 Tulane Avenue New Orleans, Louisiana 70112 USA 504.588.2296 (fx) 504.584.3586 devildog@mailhost.tcs.tulane.edu~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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