Pepsyn: On-resin removal of SBut

From: John Wade (j.wade@hfi.unimelb.edu.au)
Date: Sat Mar 04 2000 - 22:12:34 EST

Dear All,

Does anyone have experience with, or know of, an efficient means of
removing the thiobutyl group from cysteine while the synthetic peptide
remains on the solid support? The only reference I have is Eritja et al
(Tetrahedron, 43, 2675, 1987) who used mercaptoethanol:DMF (1:1, v/v) for 5
hours at r.t. Is there a more rapid means?

Thanks in advance for any advice.

John Wade
Howard Florey Institute
Melbourne

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