>Envelope-to: lcp2@mole.bio.cam.ac.uk
>Date: Mon, 06 Mar 2000 08:16:39 -0500
>From: Mary Ann Gawinowicz <mag4@columbia.edu>
>X-Accept-Language: en
>MIME-Version: 1.0
>To: lcp2@mole.bio.cam.ac.uk
>Subject: ZipTip
>
>Len,
>
>I'm just starting to work with nanospray needles and haven't become
>expert yet. I found that the needles containing ziptip'd digests would
>begin to spray, then clog up almost immediately. However, the situation
>improved dramatically when instead of washing the ziptip bound peptides
>5-6 times, I washed 20 times. Even the slightest amount of salt will
>clog those needles. Could this be part of the problem?
>
>Mary Ann
>--
>* * * * * * * * * * * * * * * * * * * *
>Mary Ann Gawinowicz, Ph.D.
>Columbia University/HHMI
>Protein Core Facility
>College of Physicians & Surgeons, 16-424
>630 W. 168th St.
>New York, NY 10032
>
>Phone: 212-305-3631
>Fax: 212-305-1599
>Email: mag4@columbia.edu
>
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html
This archive was generated by hypermail 2b29 : Fri Mar 10 2000 - 09:45:23 EST