trypsin digest on PVDF

From: Mark W. Crankshaw (crank@pcg.wustl.edu)
Date: Mon Mar 06 2000 - 12:26:55 EST

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Hello! What is a current protocol for trypsin digest of a Coomassie Blue
R-250 stained protein on PVDF membrane? We have been doing only in situ
gel piece
work for so long, I have not stayed current on the PVDF side. Thanks, Mark.

Mark W. Crankshaw
Protein and Nucleic Acid Chemistry Laboratories
Department of Molecular Biology and Pharmacology
Washington University School of Medicine
660 South Euclid Avenue, Campus Box 8103
St. Louis, MO 63110 USA

voice: (314) 362-0285
FAX: (314) 362-4698
email: crank@pcg.wustl.edu
web: http://pharmdec.wustl.edu/pnacl.home.html

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