Bernard, I have tried pop5 on my only 3700 with poor results so far. We are
getting 720-780 really great reads with hardly any miscalls on pop6. Pop5
gave poor resolution, out to only 550-600 bases before N calls appeared and
the first 50-70 bases were "compressed" as you have seen. Some people are
lowering the cuvette temperature with good success, others are raising the
cuvette temperature with good success. I haven't tried that yet mostly
because i only have one instrument and a lot of samples to process, so doing
R&D on the machine is hard to fit in. I have tried lowering the voltage on
pop5 to 120v/cm with not much success either. I might be able to try pop5
this weekend, depending on whether the urge to go skiing wins over pop5! For
now I'm sticking with Pop6 and waiting to see what comes out of the genome
community with pop5.
Margaret Robertson
Director, DNA Sequencing Facility
University of Utah,
4A 432A, SOM
50 North Medical Drive,
Salt Lake City, UT 84132
Tel: 581-4736
Fax: 585-2978
margaret.robertson@hci.utah.edu
http://www.hci.utah.edu/groups/sequencing
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