Any thoughts on the use of Ziptips to possibly clean up a tryptic digest
of an electro-eluted sample from SDS PAGE? The sample was sent from Peru-
highly coloured with Coomassie and I now need to separate the resultant
peptides on my PE ABI173 cHPLC and am loath to inject such a mess.
Any thoughts/protocols on cleanup of Coomassie from a peptide containing
liquid would be greatly appreciated.
Regards,
Suzanne Perry-Riehm
Suzanne C. Perry, Research Associate
NAPS Protein Sequencing and Peptide Mapping Laboratory
Biotechnology Department, University of British Columbia
CANADA
phone:(604)822-9688( direct) or (604)822-9662 (main) Fax:(604)822-0676
email:perry@interchange.ubc.ca
Visit our web page at : www.biotech.ubc.ca/naps/napshome.htm
This archive was generated by hypermail 2b29 : Fri Mar 10 2000 - 09:45:23 EST