RE>cellulase extraction

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                      RE>cellulase extraction 3/9/2000
Dear Tanya

I don't know anything about cellulases, but have had experience with an unstable glycosidase. Many years ago, I purified the cytosolic alpha-D-mannosidase from rat liver; it was an extremely unstable enzyme, and the only way I could stabilize and purify it was by adding the competitive inhibitor mannosylamine to all buffers.

Are there any small-molecule competitive inhibitors of cellulase that might bind to and stabilize your enzyme?

Vernon

Vernon A. Shoup
Regeneron Pharmaceuticals
Rensselaer, NY 12144

vernon.shoup@regpha.com
(518)488-6012

--------------------------------------
Date: 3/9/2000 4:41 PM
To: VERNON SHOUP
From: tcarroll
Hi everyone!

     I'm attempting to extract cellulase from peaches, but I need to
maintain the enzyme activity. I've tried using a high salt phosphate
buffer with no success.
     Does anyone have any suggestions?

                    Thanks!

                          Tanya Carroll
                          USDA - Agricultural Research Service
                          Fresno, CA USA

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