Dear Randy:
1. Already existing disulfide bridges stay intact during iodine oxidation.
2. We extract the iodine with CCl4 or CHCl3 by using a separatory
funnel. 3-5 extractions are normally sufficient. Then we lyophylize
the aqueous phase which contains the peptide.
Have fun!
Henriette
>We are planning on performing iodine oxidation in solution to form a
>disulfide bond from a peptide with 2 Cys(Acm) groups. I have 2 questions
>regarding this:
>
>1. Will the iodine treatment disturb an existing disulfide bond in the
>peptide?
>
>2. Can you share a protocol for removing the iodine after oxidation is
>complete (prior to HPLC purification)?
>
>Thanks for your help.
>
>
>Randy Wilhelm
>Sr. Research Chemist
>Mallinckrodt, Inc
>Discovery Research Peptide Team
>Phone (314)654-3965
>Fax (314)654-8911
>rrwilhe@mkg.com
Henriette A. Remmer, Ph.D
Director of Protein Services
Protein Sciences Facility at the Biotechnology Center
University of Illinois
311/315 Noyes Laboratory, Box 62-1
505 S. Mathews Ave.
Urbana,IL 61801
Tel: (217) 333-4695 (Lab) (217) 333-3841 (office)
Fax: (217) 244-1142
homepage: http://www.life.uiuc.edu/biotech/protein_sc.html
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