Dear All,
the Nucleic Acid Research Group (NARG) presented data at ABRF 2000 from
their most recent study. This study involved the synthesis of unmodified
-21M13 forward primer and various modified forms and analysis of the
products using MALDI-MS, ESI-MS and capillary electrophoresis. The
synthesis of the oligos took place in the NARG members facilities and
each type of analysis used was carried out by one lab. on the entire set
of oligos produced.
We hope that someone out there can shed light on an observation we made
during the analysis, which is puzzling us somewhat. In the case of
several oligos we made there is an observed increase in mass of +94Da
(and in some cases multiples of +94Da). This mass difference is
irrespective of the type of modification used, which lab. it was
synthesised in, the scale of synthesis, the source of reagents and was
consistently detected by both methods of mass analysis used. All oligos
were synthesised using standard phophoramidite chemistry, deprotected
for 10-12 hours in ammonia and were anlysised without prior desalting.
Any suggestions to what may be causing this observation will be
gratefully received.
Kathryn Lilley
NARG members
Martha Gunthorpe (chair)
Jay Fox
Karl Hager
Kathryn Lilley
Mark Lively
Stephen Scaringe
Anthong Yeung
Dr Kathryn Lilley
Laboratory Manager
Protein and Nucleic Acid Chemistry Laboratory
University of Leicester
Lancaster Road
Leicester LE1 9HN
tel 44 116 252 5613
fax 44 116 252 5616
email ksl@le.ac.uk
This archive was generated by hypermail 2b29 : Mon Mar 27 2000 - 11:29:46 EST