I run a sequencing core and have recently had several colleagues
issue the same complaint.
Their complaint is centered around not reading close enough to the primer.
Several of them have stated that in the past we were giving back data within
10 bases of the primer. Unfortunately, the past 2-3 weeks worth of data has
only given data 50 bases from the primer.
I'm suspecting our purification method, but I would love suggestions.
20ul reaction with Big Dye (1/2 rxn)
Purification with Edge Biosystems 96 well gel filtration blocks
Speedvac to dryness
rehydrated in blue dextran/formamide
load 2/3 of the rehydrated volume.
Chris Jacobs
DNA Sequencing Lab
Parke-Davis Pharmaceuticals
Office Phone: (734) 622-5724
Lab Phone: (734) 622-1815
E-mail: chris.jacobs@wl.com
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