Wait, wait wait.... I need more info.
So it's not a dipeptide, it's a protein, right? And only one protein you have found does this? And the protein is pure?
Is cleavage pH sensitive?
Tom Andersen
__________________
Thomas T. Andersen
Protein Chemsitry Core FAcility
Albany Medical College
Albany, NY 12208
518 262-5368
anderst@mail.amc.edu
HELP
Mechanism gurus/Organic Chemistry mavens,
I have observed cleavage at the QT bond in a protein following long (5 to
10 days) incubation in sterile 15mM Tris pH 8 at room temperature. Product
was massed and also gave the correct N-terminal sequence. Reaction has
been performed more than once with different batches of buffer and stock
preparations of protein. Any thoughts on what might be going on???
Thanks,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu
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