Dear Panagiotis,
Try the following reference.
WARD, LD, REID, GE, MORITZ, RL, SIMPSON, RJ
STRATEGIES FOR INTERNAL AMINO-ACID-SEQUENCE ANALYSIS OF PROTEINS SEPARATED
BY POLYACRYLAMIDE-GEL ELECTROPHORESIS
JOURNAL OF CHROMATOGRAPHY
P 199 - 216
PY 1990
PD OCT 19
VL 519
IS 1
Best of luck.
Robert Moritz
Chief Research Officer
Joint Protein Structure Laboratory,
Ludwig Institute for Cancer Research,
P.O. Box 2008, Royal Melbourne Hospital
Parkville, Victoria,
Australia 3050
Phn +61-3-9341-3155
Fax +61-3-9341-3192
-----Original Message-----
From: Panagiotis Stasinakis [mailto:pstasina@mail.pasteur.gr]
Sent: Monday, March 20, 2000 2:55 AM
To: Recipients of ABRF List
Subject: elution from PVDF or NC
Hi there!!!
I am wondering if is possible to elute a protein from a PVDF or NC
(nitrocellulose) membrane. If there is a specific way or protocol i would pe
grateful if you could inform me about this.
Thanks!!!
Panagiotis Stasinakis
Phd student
Hellenic Institute Pasteur
Departement of Biochemistry
GR 11521, Athens
Greece
pstasina@mail.pasteur.gr <mailto:pstasina@mail.pasteur.gr>
pstasinar@mailhost.bio.auth.gr <mailto:pstasinar@mailhost.bio.auth.gr>
+030 01 6478840
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