Millie,
I have (successfully) prepared 14-mer with Cys(farnesyl)-COOCH3 a
year ago by following a published procedure: M.Koppitz et al. Int.J.Peptide
Protein Res. 48. 1996, 377-390.
It involved a)preparing of the fully protected (with N-term Fmoc
ON) peptide fragment on a chloro-Trityl resin, b)condensation of the
cleaved, fully protected fragment (w/o any futher purification) with
H-Cys(trt)-COOCH3, c) cleavage and prep. HPLC purification of the
Fmoc-peptide-Cys(SH)-COOCH3 product, d) farnesylation with farnesyl bromide
(DIEA/DMF) and subsequent Fmoc removal with piperidine in the one-pot
reaction, e) HPLC purification of the final product in ACN gradient with
both solvents containing 15mM AcONH4 (pH ~6).
FYI, my peptide did not contain any basic amino acids (R,K,H), which would
(presumably) make this preparation more complex.
Hope this helps in your endeavor.
Jan Lukszo
At 01:37 PM 3/17/00 -0500, you wrote:
>
>
>A customer wants a synthetic peptide with Cys (farnesyl)-COOCH3 as the
>C-terminus. Does anyone know whether it can be made on a synthesizer, or
>post-synthesis modification?
>
>Thanks in advance.
>
>Millie McAdams
>HHMI Biopolymer Facility @ DUMC
>919-684-2652
>919-684-5458
>mcada002@mc.duke.edu
>
>
>
>
Dr. Jan Lukszo
Peptide Analysis and Synthesis Unit
NIH/NIAID/OSD/SBS
Twinbrook II
12441 Parklawn Drive
Rockville, MD 20852
phone:(301)496-3786
fax:(301)480-2618
E-Mail: lukszo@ncisun1.ncifcrf.gov
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