Dear ABRF' Members
I am currently using TFA-aminolinker (C6) to couple to oligos and
further conjugation with NHS esters.
I am a bit dissapointed talking about the profile I get using anion
exchange HPLC. Something like 4 or 5 peaks for easy oligos (dT16, 18-20
mers heteropolymers) and a bit difficult to know where the expected
aminated compound is. Anyway the conjugation reaction with NHS ester
works fine
I suspect some post synthesis side reactions with the amino linker .
Should I use instead MMT-aminolinker ? Does the amino C12 linker work
better ?
Thank for your help
Regards
Alain
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Alain LAURENT Ph. D
DNA&Peptide Chemistry
ESGS Groupe CYBERGENE
11, rue Claude Bernard
35 400 Saint MAL0
FRANCE
Tel: +33 (0)2 99 21 90 40
Fax: +33 (0)2 99 21 90 41
e-mail: a.laurent@eurosequence.com
http://www.eurosequence.com
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