David
One option is to set the HPLC up with a switching valve(like a rheodyne) and
then just toggle it between cap/anal lc work-you can leave the splitter in
place that way. The ABI may have much less gradient delay than the Finnigan
pump (30-80 minutes) and so you may be happier with that however.
Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software
-----Original Message-----
From: Chin, David T. <ChinD@missouri.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Thursday, March 23, 2000 1:11 PM
Subject: MS TSQ's HPLC
>The TSQ's HPLC is an "analytical" HPLC for std. 2 mm column. It's the OEM
>from the company. I want to run both capillary and analytical columns with
>the TSQ, but have limited resources ($) and have an extra ABI 120. Is it
>better to use a splitter on the TSQ OEM HPLC and keep swapping it back and
>forth, or have the ABI 120 with splitter on a cart and swap out the entire
>HPLC?
>
>David
>
>David T. Chin
>Director, Protein Core Facility
>Protein Chemistry and Expression
>2-17 Agriculture Building
>Univ. of Missouri - Columbia
>Columbia, MO 65211-7170
>
>SHIPPING AND ACTUAL LOCATION:
>2-17 Agriculture Building (office)
>2-31 Agriculture Building (Lab)
>
>
>e-Mail: chind@missouri.edu
>web: http://www.biotech.missouri.edu/pc
>[mailto:chind@missouri.edu]
>Phone: 573-882-2027
>Fax: 573-882-7105
>
>
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