Dear David:
I'd suggest to use the splitter on the ABI, but make sure that you bypass
the mixer to reduce the dwell volume -- otherwise you might be waiting for
the gradient to reach your column for quite some time.
With this set-up you'd have a dedicated capillary and a dedicated 2.1 mm
set-up. You can even add a capillary flow cell to the ABI 120 which would
allow you to even have a UV trace -- and is also helpful in trouble shooting
(are there peaks visible in the UV the problem should be downstream from
there, are there no peaks the problem is with the LC)
Please contact me directly if you'd like "commercial" information.
Best regards: Jean-Pierre
----------------------------------------------------
Jean-Pierre Salzmann
Project Manager
LC PACKINGS (USA) INC
80 Carolina Street
San Francisco, CA 94103
Phone 415-552-1855
Fax 415-552-1859
e-mail jps@lcpackingsusa.com
www.lcpackings.com
----------------------------------------------------
----------
>From: "Chin, David T." <ChinD@missouri.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: MS TSQ's HPLC
>Date: Thu, Mar 23, 2000, 8:33 AM
>
>The TSQ's HPLC is an "analytical" HPLC for std. 2 mm column. It's the OEM
>from the company. I want to run both capillary and analytical columns with
>the TSQ, but have limited resources ($) and have an extra ABI 120. Is it
>better to use a splitter on the TSQ OEM HPLC and keep swapping it back and
>forth, or have the ABI 120 with splitter on a cart and swap out the entire
>HPLC?
>
>David
>
>David T. Chin
>Director, Protein Core Facility
>Protein Chemistry and Expression
>2-17 Agriculture Building
>Univ. of Missouri - Columbia
>Columbia, MO 65211-7170
>
>SHIPPING AND ACTUAL LOCATION:
>2-17 Agriculture Building (office)
>2-31 Agriculture Building (Lab)
>
>
>e-Mail: chind@missouri.edu
>web: http://www.biotech.missouri.edu/pc
>[mailto:chind@missouri.edu]
>Phone: 573-882-2027
>Fax: 573-882-7105
>
>
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