Pepsyn: Recleavage of PEG resin

From: RKrishnan@coh.org
Date: Mon Mar 27 2000 - 12:18:29 EST


Hi ABRFers,
     I have noticed that I need to recleave our PEG resin to maximise our
     peptide yeilds. The recleavage products do not look bad compared to
     the products of the first cleavage. I was wodering if anybody has
     similar experience, or is it common practise? We use PEG-PS resins
     from PE Bio, already loaded with our C-terminal amino acids. I
     normally use cocktail K first time around and cocktail R for the
     second time.
     Thanks for any input.
     Radhika.
     City of Hope, Duarte, CA



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