protein load. Absolute vs relative

From: jaime paba martinez (bucaros@unb.br)
Date: Mon Apr 03 2000 - 11:01:51 EDT


Hello Everyone,

I am willing to monitore protein expression of a
parasite during its differentiaton cycle. The problem
is that the protein content of each stage is very
different because of the morphological changes. My
doubt is if i should load my gels with the same
quantity of proteins or with the same number of
parasites in order to look for different expression.

Any help is appreciated,

Jaime Paba
Universidade de Brasilia
Brazil



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