Keri:
Our facility has had some success sequencing hairpins using either
dGTP or dRhodamine. dRhodamine requires longer extension times (at
least 4 minutes @ 60 deg.) and more cycles (I use 60). I have some
of my better successes on my website.
www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/hairpin.html
www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/gc_gt70.html
I would be glad to provide more detail if you wish. Hope this helps!
Sheryl Christofferson
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Oklahoma Medical Research Foundation
DNA Sequencing Facility
Oklahoma City, Oklahoma
(405) 271-7393 ext. 34565
www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/
sherylc@omrf.ouhsc.edu
> Does anyone know how to sequence or PCR a hairpin loop structure?
>
>
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