Carmen,
I don't know of many labs who use this procedure anymore, but when I was at Purdue, the agricultural station used to do it routinely (this was 12 yrs ago). You may be able to get the # by contacting the biochem dept (765-494-1600). I don't know if they do contract work but they may know of someone who does. At GI we use amino acid analysis as a backup method (either Beckman or Picotag).
I once used a method (it worked!) for determining total nitrogen of affinity chromatography supports that should work fine and is easier than the Kjeldahl. I don't have the exact reference, but it was on page 9 of Meth. Enz. volume titled Affinity Chromatography. I attached the scanned page with the procedure for you, I hope you can read it.
Tom
***********************************************
Thomas J. Porter, Ph.D.
Principal Scientist
Biopharmaceutical Characterization
and Analysis
Genetics Institute
1 Burtt Road
Andover MA 01810
978-247-2166 (voice)
978-247-2837 (fax)
tporter@genetics.com
>>> <Carmen_Young@Biogen.com> 04/11 7:21 PM >>>
Hello All
We are trying to obtain an extinction coefficient for several proteins and
would like to be able to use the Kjeldahl analysis method as a backup procedure.
Can anyone provide me with the name, location and phone number for laboratories
(contract lab) that are currently performing this analysis .
Thanks,
Carmen Young 617-679-2192
Biogen Inc.
Structural Biochemistry
14 Cambridge Center
Cambridge MA 02142
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