The dimer is most likely a result of MALDI mass spectrometry analysis, and commonly observed for proteins, especially in concentrated samples.. You were correct in trying to find the triply charged dimer peak; it's absence, although not constituting conclusive proof, would support the notion that the dimer is, indeed, formed in the mass spectrometer during ionization. However, as far as I know, short of doing size exclusion chromatography (or, perhaps, non-denaturing PAGE) you can not say with absolute certainty that the dimer is, indeed, a feature (aka artifact) of mass spectrometry.
Ioannis Papayannopoulos
---------------------------------
Do You Yahoo!?
Send online invitations with Yahoo! Invites.
This archive was generated by hypermail 2b29 : Thu Apr 20 2000 - 14:46:39 EDT