Well, this is my fault. When I first posted the question, I spelled
"dimer," after the spell check, I went along with the suggestion from
Netscape of "dimmer," sorry for the typo. Thank you all for the helpful
responses.
Hong
"Amina S. Woods" wrote:
> Dear all:Before I get delued with mail about my spelling skills, I
> would like to apologize and confess to all of you that I have always
> scored a grade of zero in dictation in French (my first lanuage) and
> the other languages I've learned (English,Hebrew,Spanish and Arabic).
> Have a good Science week.AminaAmina S. Woods, Ph.D.NIDA Intramural
> Program, NIH5500 Nathan Schock DriveBaltimore, MD 21224Tel:
> 410-550-1507Fax: 410-550-2971e-mail: awoods@intra.nida.nih.gov
>
> -----Original Message-----
> From: neil brew [mailto:neil.brew@bbsrc.ac.uk]
> Sent: Monday, April 17, 2000 4:15 AM
> To: 'Amina S. Woods'
> Subject: RE: MALDI: Protein dimmer
>
> Surely you mean a dimer?
>
> -----Original Message-----
> From: Amina S. Woods [mailto:awoods@jhmi.edu]
> Sent: 15 April 2000 17:16
> To: Recipients of ABRF List
> Subject: RE: MALDI: Protein dimmer
>
> I do a lot of work at pH of 5-7 to study
> non-covalent interaction by MALDI
> (Enzyme-Substrate complexes, DNA-peptide etc...).
> So I have to use matrices with pH in the range of
> 5-7. In those cases I do see more dimmer
> formation than usual even at low concentrations of
> compound deposited. Usually if I look at the same
> sample using matrices at pH less than 2.0 these
> dimmers are absent. I do believe that these
> dimmers are usually two molecules trapped
> together. I have also unpublished data using basic
> matrices showing 5 isomers (MH+) as well as the
> MH2+. If you are interested in non-covalent
> interaction I'll refer you to my papers and others
> on the subject. I think the best paper to read is
> Terry Farmar's review in the Journal of Mass
> Spectrometry. I think it was published in 1998.
> Akos Vertes and John Callahan published in 1995,
> and Louis Pannell also published in 1995 on
> non-covalent interaction seen by MALDI Amina S.
> Woods, Ph.D.NIDA Intramural Program, NIH5500
> Nathan Schock DriveBaltimore, MD 21224Tel:
> 410-550-1507(office)Tel:410-550-5168 (lab)Fax:
> 410-550-2971e-mail: awoods@intra.nida.nih.gov
>
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