For c-terminal specific biotinylation, check Methods in Enzymology v.184
p.160 I hope it helps.
Andy
------------------------------------------------------------------------
Andy Ng
Department of Biochemistry
McGill University
Email: andy.ng@BRI.NRC.CA
Phone: 514-496-8010
514-496-6332
On Mon, 17 Apr 2000, Aki Hoji wrote:
> Dear,
>
> Does anybody know strategies to selectively biotinylate the amino acid in
> carboxy terminus of a given protein. Any help will be appreciated.
>
>
> regards,
>
>
>
> Aki Hoji
> University of Pittsburgh
> Department of Infectious Diseases and Microbiology
> Rm522 Parran Hall, GSPH-IDM
> 130 Desoto Street, Pittsburgh PA 15261
> tel: (412) 624-3072/0776
> fax: (412) 624-4953
>
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