Lys-C substrate source

From: John Hempel (hempel@psc.edu)
Date: Thu Apr 20 2000 - 15:33:59 EDT


In-gel digesters:

        Would anyone have a US source for
N-(alpha)-benzoyl-DL-lysine-p-nitroaniline (most likely hydrobromide)?
It's available from WAKO but not stocked in the US, thus at least a 3wk
delay they say.

Bachem and Sigma come up empty as does Calbiochem, altho Calbiochem has
N-(alpha)-Boc-L-lysine-thiobenzyl ester which would presumably serve as a
substrate for Lys-C, but without knowing the relative rates of the two
substrates I'd prefer to stick with what WAKO standardizes their preps with.

Thanks,
JH
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Clapp Hall 301
Pittsburgh PA 15260 USA

email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html



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