Katheryn,
According to Chapter 2 in "Proteome Research: New Frontiers in
Functional Genomics", "...not more than 20% of the expressed
genes of mammalian cells (5,000 proteins corresponding to 10,000
to 15,000 polypeptides or isoforms) are detectable...". Typically, of
the genetically expressed proteins, ~30% are phosphorylated. I
will need to look further into the reference to see if there is any
more pertinent information.
Damon
On 20 Apr 00, at 10:59, Katheryn Resing wrote:
> Hi, Does anyone know of a reference (or have an estimate themselves), of
> the amount of global post-translational modification occurs in a typical
> mammalian proteome? That is, if we have 3500 "spots" on a 2D gel, can we
> estimate how many different proteins that represents?
>
> Thanks in advance for any help.
>
> Katheryn Resing
>
>
Damon C. Barbacci, Ph.D.
Stanford Research Systems
1290 D Reamwood Ave.
Sunnyvale, CA 94089
Phone: (408) 744-9047 ext 249
Fax: (408) 744-9077
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