You should know better than most,being at Genzyme.I have been working with
hCG for almost
25 years,and,I have yet to find a method,which will handle glycoprotein,in
a satisfactory
manner.Just try to do mass spec with a glycoprotein,which has over 20%
carbohydrate.
"Zhang, Kate" <Kate.Zhang@Genzyme.com>@aecom.yu.edu> on 04/20/2000 10:38:07
AM
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To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc: "Vanpatten, Scott" <Scott.Vanpatten@Genzyme.com>
Subject: Protein-chip technology
Hello, everyone,
Has anybody ever look into the new proteinchip technology, for example,
Ciphergen? How does it compare with the conventional 2D gel plus mass spec.
approach in real sample for protein discovery? As far as I can tell, I am
not at all convinced by what they claim for protein identification after
protein capture? First they will identify the protein from accurate
molecular weight determination-----I am not sure. Then , they will do all
kinds of enzymatic digestion or other treatment such as deglycosylation on
the spot, and run MALDI for protein identification---I am not sure either.
They even claim for quantitative analysis????
Can anyone give me some education on this? Thanks in advance.
Kate Zhang
Xiaokui Kate Zhang, Ph.D
Genzyme Corp.
1 Mountain Rd.
Framingham, MA 01701
Tel: 508-270-2294
Fax: 508-872-9080
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