Dear Alex !
I would like to know, in which aspect of protein-protein interactions you
are particularly interested:
Do you want to identify interacting partners ? (1)
Or do you intend to map interaction sites of known protein complexes ? (2)
Or are you aiming at quantifying the strength of interactions ? (3)
Besides the well-established approaches in molecular biology, there are a
lot of mass-spec- related tools around:
(1) Native PAGE + MALDI-MS / ES-MS/MS identification of proteins in
complexes.
(2) Chemical cross-linking (possibly plus PAGE) / surface-labelling /
limited proteolysis
plus MS peptide mapping.
(3) MS plus SPR (BIAcore): MS identifies the interacting partners, whereas
the Surface-Plasmon-Resonance technique allows for quantification of the
bound partner (Kd determination).
In general, the mass-spec tools are good in terms of sensitivity: it is
possible to identifiy interacting partners and to (partially) map their
contact sites from amounts corresponding to silver-stained gel bands.
On the other hand, data interpretation is complex and quite time-consuming.
However, I would like to learn more about the Cyphergen technology as well
!
These are just a few "spontaneous" thoughts.
Best regards,
Martin.
P.S.: I completely agree with the idea to distribute questions AND answers
among our "community". I think, we can encourage more people doing so by
maintaining a cultivated and friendly atmosphere of discussion.
*****************************
Martin Kussmann, PhD
R&D, Protein Analysis /
Mass Spectrometry
CERBIOS-PHARMA S.A.
Via Pian Scairolo 6
CH - 6917 Barbengo (Lugano)
Switzerland
Phone: ++41 - 91 - 985 63 11
Fax: ++41 - 91 - 985 63 25
E-mail: rdbio2@cerbios.ch
-----Original Message-----
From: alex bell [SMTP:ehjb@musica.mcgill.ca]
Sent: lunedi, 24. aprile 2000 20:04
To: Recipients of ABRF List
Subject: protein protein interactions
Dear All,
I am interested in surveying techniques/technology related to
"protein-protein interactions".
The Ciphergen technology seems to me to be the newest/hottest thing out
there.
What else is there and how do these measure up with respect to new
technology/interactions and also of course sensitivity?
Anything will be muchly appreciated.
Thanks in advance
alex
Alexander W. Bell
Anatomy and Cell Biology
McGill University
3460 University Street
Montreal Quebec H3A 2B2 Canada
phone (514)398-1393
fax (514)398-5047
email ehjb@musica.mcgill.ca
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