Long time ago,phthalic anydride was used.The reason for this was to produce
a number of
acidic peptides.This was in the days of classical(not solid phase) peptide
synthesis and
ion exchange purification as a first step.Phthaloylation was also done in
solid phase
synthesis.I tried the procedure a number of times,and results were not too
good.And,hardly
anyone uses capping in Fmoc chemistry,when it should be used..
Fuselier de la Claire <devildog@mailhost.tcs.tulane.edu>@aecom.yu.edu> on
04/25/2000 04:44:34 PM
Please respond to devildog@mailhost.tcs.tulane.edu
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Subject: Pepsyn: capping strategies
Has anyone had any luck using something besides acetic anhydride to cap
the unreacted amines? Perhaps something hydrophobic that will change the
retention time significantly. Thanks in advance...
Joseph
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Joseph A. Fuselier Peptide Research, SL12 Tulane University School of Medicine 1430 Tulane Avenue New Orleans, Louisiana 70112 USA 504.588.2296 (fx) 504.584.3586 devildog@tulane.edu~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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