Dear All,
I am interested in mapping protein-protein interactions
in a cell after an external stimulus. My idea is to
expose the whole cells to chemical crosslinking, to make
cell disrutpion and follow to the protein separation
through 2D electrophoresis. My questions:
- would IPG gels support high mass oligomers? If not,
IEF gels should be better?
- Which crosslinker should I use that enters the cell
and that does not crosslink everything.
- would be better using a chromatographic separation
rather than electrophresis due to the high masses in
question?
Any suggestion will help.
Thanks in advance!
Jaime Paba
Centro Brasileiro de Sequenciamento de Proteinas
Brazil
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