At 11:02 26.04.00 -0400, you wrote:
>I too have received samples on nitrocellulose and been told they are on
>PVDF. Our clean up was not as easy and required PE Biosystems to replace
>out every block and line in our 477. Needless to say I now test every
>"PVDF" sample we receive in neat TFA even if it means a loss of a portion
>of sample. Nitrocellulose will disintegrate in neat TFA and PVDF remains
>intact sometimes with slight discoloration depending on the brand of PVDF.
>I recommend this simple test to any one routinely providing Edman
>sequencing on PVDF blotted samples. It can save you a major clean up and
>repair job.
>
>Cheryl
Another very simple method to verify if it's PVDF or nitrocellulose is to
add a drop of acetone to a tiny piece of membrane. Nitrocellulose will
easily dissolve within seconds. By accident, I have used a NC-membrane in
our 473A once as well, but thouroughly cleaning all the affected lines with
acetone helped. Just the column had to be exchanged.
Yours sincerely,
Marcus
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Dr. Marcus Macht
Universit”t K–ln
Zentrum f¸r molekulare Medizin
Zentrales Servicelabor
Joseph-Stelzmann-Str. 52
50931 Koeln
Tel.: +49 221 478-6995
Fax: +49 221 478-6977
e-mail: Marcus.Macht@uni-koeln.de
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