Hello everyone,
We would like to synthesize a short peptide containing two N-metylated
residues. Ser-Val-MetAsp-Val-MetGlu-Y.
The first attempt with the use of 433A synthesizer, with 0.1mM FastMoc
MonPrevPeak chemistry ( three deprotection loops) gave us only a few mgs
of the product. The conductivity tracing looked OK.
Any advices on the different coupling reagents, manipulation of
deprotection/coupling cycles, better (lower substitution?) resin
would be greatly appreciated.
Thanks in advance.
Krystyna
Dr. Krystyna B.Piotrowska
Protein Service Laboratory, NAPS
Biotechnology Laboratory
Room 237, Wesbrook Building
6174 University Boulevard
University of British Columbia
Vancouver B.C., V6T 1Z3
Canada
Tel. (604) 822 9662, Fax (604) 822 0676
www.biotech.ubc.ca/naps/napshome. htm
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