**************************************
Alain LAURENT Ph. D
DNA&Peptide Chemistry
ESGS Groupe CYBERGENE
11, rue Claude Bernard
35 400 Saint MAL0
FRANCE
Tel: +33 (0)2 99 21 90 40
Fax: +33 (0)2 99 21 90 41
e-mail: a.laurent@eurosequence.com
http://www.eurosequence.com
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attached mail follows:
Hi Trudy and Terry
The Pharmacia Spin columns work very well indeed for desalting small
quantities of oligos. For larger ones the NAP 5,10, 25 should be better. The
protocol is very easy. Have a look on the Pharmacia catalogue. They tell you
which one you have to use depending on your proper application.
You cannot compare the purification done with an OPC cartdrige and sephadex.
In the first case (and if you run a DMTON synthesis) you can separate some
failures sequences from the expected DMTOn compound and depending on the
further elution step you can desalt the oligo. With sephadex most of the
failure sequences (except the very short ones < 8 mers) go throw the column
and coelute with the expected compound. But the desalting step is very
efficient since all the small molecules are trapped inside the resin bed .
Biorad sells as well desalting columns for oligo (P2...if I remember)
Hope this help
Alain
"Holyst, Trudy" wrote:
> Hi All,
> We are interested in desalting oligos using Sephadex spin columns but are
> not sure what Sephadex or protocol to use. Also, is there any difference in
> the yield or quality of the products compared to OPC purified oligos?
>
> Thanks in advance for your response,
>
> Trudy Holyst
> Terri Fletcher
>
> Blood Research Institute
> Peptide Core Laboratory
> Blood Center of SE WI
> Milwaukee WI 53201
--************************************** Alain LAURENT Ph. D DNA&Peptide Chemistry ESGS Groupe CYBERGENE 11, rue Claude Bernard 35 400 Saint MAL0 FRANCE Tel: +33 (0)2 99 21 90 40 Fax: +33 (0)2 99 21 90 41 e-mail: a.laurent@eurosequence.com http://www.eurosequence.com **************************************
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