Dear Nicola,
We have sucessfully silver stained our glycerol-containing 2D-gels (typically, t
he ones that you used for gradient gels). I would
rather suspect your silver nitrate solution. And, by the way: you are usually mu
ch better off silver staining your gels with your
own chemicals. I typically get better results plus I can quality control which c
hemicals I use.
Axel Ducret
Nicola O'Reilly wrote:
> Dear All
>
> We've had problems staining our 2-D gradient gels with the Owl kit. No
> markers or spots appear. We've tried making our own stain using a modified
> Schevchenko protocol and that's worked on the same gels . We're wondering
> whether the problem with the Owl kit has anything to do with using glycerol
> to make the gradient gels . Has any one seen the same effect or has any
> advice?
>
> Thanks for your help.
>
> Nicola O'Reilly
>
> Nicola O'Reilly
> Peptide Synthesis Laboratory & 2-D Gel Electrophoresis Laboratory,
> Imperial Cancer Research Fund.
> 44 Lincoln's Inn Fields, London, WC2A 3PX.
> Tel 020 7269 3318 or 020 7269 2886
> Fax 020 7269 3579
-- ------------------------------------------------------------------------------ ------------------------------------------------------Axel Ducret, Ph.D. Senior Research Biologist Merck-Frosst Canada Inc. Dept. Biochemistry and Molecular Biology P.O. Box 1005 Pointe-Claire-Dorval PQ H9R 4P8 Canada
tel. + (514) 428-3428 fax + (514) 428-4900
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