Hi Jenny,
When I want to form dimers I dissolve the lyophilized peptides in 20-30%
DMSO in 5mM ammonium acetate, pH 5-7. I run samples using the HPLC and watch
for peak shifts. This is essentially the procedure outlined by Tam et al in
the J Am Chem Soc 1991. 113, 6657-6662.
Hope this helps.
Trudy Holyst
Blood Research Institute
Peptide Core Laboratory
Blood Center of SE WI
Milwaukee WI 53201
-----Original Message-----
From: Jenny Shipway [mailto:jennys@biols.susx.ac.uk]
Sent: Thursday, May 04, 2000 7:18 AM
To: Recipients of ABRF List
Subject: swapping disulphides to equilibrium
Hi there,
I've got a couple of 30aa peptides, each with a C-terminal cyteine
residue. These easily oxidise to form dimers. What I want to do is to
oxidise them to form an equilibrium mix of homo- and hetero-dimers,
giving them as much chance to swap about as possible. I've previously used
glutathione as a 'swapping agent', but really want something that isn't
going to end up stuck to my peptides, as that messes up my HPLC analysis
of the resulting mix.
Any ideas?
Jenny Shipway
University of Sussex
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