At 01:17 PM 5/4/2000 +0100, Jenny Shipway wrote:
>
>Hi there,
>
>I've got a couple of 30aa peptides, each with a C-terminal cyteine
>residue. These easily oxidise to form dimers. What I want to do is to
>oxidise them to form an equilibrium mix of homo- and hetero-dimers,
>giving them as much chance to swap about as possible. I've previously used
>glutathione as a 'swapping agent', but really want something that isn't
>going to end up stuck to my peptides, as that messes up my HPLC analysis
>of the resulting mix.
>
>Any ideas?
>
>Jenny Shipway
>University of Sussex
>
>
>
>
Jenny,
Give DTTox (oxidized DTT) a try. I don't think you should not end up with
any "mixed-disulfides" = thiol reagent/peptide. Calbiochem sells DTTox.
Regards,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu
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